Journal: Medical Science Monitor

Article Title: Helicobacter Pylori Induces GATA3-Dependent Chitinase 3 Like 1 (CHI3L1) Upregulation and Contributes to Vascular Endothelial Injuries

doi: 10.12659/msm.916311

Figure Lengend Snippet: Figure 3. The expression of GATA3 and CHI3L1 were elevated in the Helicobacter pylori infected mice model. (A, B) Immunohistochemical staining was used to demonstrate the upregulation of GATA3 and CHI3L1 in mice thoracic aorta after being gavaged with CagA+ H. pylori or CagA– H. pylori. (C, D) Western blot used to show the expression of GATA3 and CHI3L1 protein of the aforementioned mice thoracic aorta, b-asctin levels were used as a loading control. * P<0.05, ** P<0.01, *** P<0.0001 **** P<0.0001 versus NC group. # P<0.05 versus CagA– group. CagA – cytotoxin-related protein.

Article Snippet: It was then incubated using the primary antibodies of anti-GATA3 rabbit polyclonal antibody (1: 1000, Cusabio, China), the anti-CHI3L1 rabbit polyclonal antibody (1: 600, Cusabio, China), the anti-p38 rabbit polyclonal MAPK antibody (1: 1000, Cell Signaling Technology, USA), the anti-p38 rabbit polyclonal phospho-p38 MAPK antibody (1: 1000, Cell Signaling Technology, USA), the anti-calnexin rabbit polyclonal antibody (1: 1000, Cell Signaling Technology, USA) and the anti-b-actin rabbit polyclonal antibody (1: 1000, Cell Signaling Technology, USA) at 4°C with gentle shaking, overnight.

Techniques: Expressing, Infection, Immunohistochemical staining, Staining, Western Blot, Control

Journal: Medical Science Monitor

Article Title: Helicobacter Pylori Induces GATA3-Dependent Chitinase 3 Like 1 (CHI3L1) Upregulation and Contributes to Vascular Endothelial Injuries

doi: 10.12659/msm.916311

Figure Lengend Snippet: Figure 4. Small interfering RNA (siRNA)-mediated knockdown of GATA3 lowered Helicobacter pylori -induced expression of CHI3L1 and p38 phosphorylation (A) Western blot shows the expression of GATA3 and CHI3L1 protein in HUVECs after transfected with siRNA-control or siRNA-GATA3 for 24 hours and cultured with medium added with CagA– H. pylori or CagA+ H. pylori or PBS for 48 hours. Calnexin levels were used as a loading control. (B) The expression of GATA3 was significant reduced by siRNA- GATA3 transfected. (C) The elevated expression of CHI3L1 caused by H. pylori was relatively decreased as GATA3 was knocked down. (D) The H. pylori-induced activation of p38 MAPK was attenuated in GATA3 knocked-down cells. * P<0.05, ** P<0.01, *** P<0.0001 versus si-control group. HUVECs – human umbilical endothelial cells; CagA – cytotoxin-related protein; PBS – phosphate-buffered saline MAPK – mitogen-activated protein kinase.

Article Snippet: It was then incubated using the primary antibodies of anti-GATA3 rabbit polyclonal antibody (1: 1000, Cusabio, China), the anti-CHI3L1 rabbit polyclonal antibody (1: 600, Cusabio, China), the anti-p38 rabbit polyclonal MAPK antibody (1: 1000, Cell Signaling Technology, USA), the anti-p38 rabbit polyclonal phospho-p38 MAPK antibody (1: 1000, Cell Signaling Technology, USA), the anti-calnexin rabbit polyclonal antibody (1: 1000, Cell Signaling Technology, USA) and the anti-b-actin rabbit polyclonal antibody (1: 1000, Cell Signaling Technology, USA) at 4°C with gentle shaking, overnight.

Techniques: Small Interfering RNA, Knockdown, Expressing, Phospho-proteomics, Western Blot, Transfection, Control, Cell Culture, Activation Assay, Saline

Journal: BMC Musculoskeletal Disorders

Article Title: The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis

doi: 10.1186/s12891-021-04043-9

Figure Lengend Snippet: Western blot analysis of human cartilage tissue, A: Western blot band map of each protein, B-F: quantitative histogram of protein bands for the expression of Cbfβ, MMP-13, IL-1β, COMP, and YKL-40 (***: p < 0.001)

Article Snippet: Cbfβ (Cat. No. bs-6984R), MMP13 ( Cat.No.bs -10250R), IL-1β ( Cat.No.bs -20448R), YKL-40 ( Cat.No.bs -10215R), and COMP ( Cat.No.bs -10286R) in the cartilage tissues were measured (above antibodies were all purchased from Bioss Biotechnology Co., Ltd., Beijing, China).

Techniques: Western Blot, Expressing